Vitamin B12 Auxotrophy in Isolates from the Deep Subsurface of the Iberian Pyrite Belt

Vitamin B12 is an enzymatic cofactor that essential for both eukaryotes and prokaryotes. The development of life in extreme environments depends on cofactors such as vitamin well. genomes twelve microorganisms isolated from the deep subsurface Iberian Pyrite Belt have been analyzed search activities require or are involved its synthesis import. Results revealed needed by these several enzymes ribonucleotide reductase, methionine synthase epoxyqueosine reductase. Isolate Desulfosporosinus sp. DEEP only genome holds a set core proteins could lead to production B12. rest dependent obtaining it oligotrophic environment which they grow. Sought import not widespread sample. dependence found supported DEEP, showing operation biogeochemical cycles